mianaz/qpcr_tool

Shiny App for quick analysis of qPCR data using ddCt method

1 good first / help-wanted issue · R · last activity Sep 12, 2025

4 stars 1 forks 4 watchers R MIT License

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1 Open Issue Need Help Last updated: Sep 12, 2025

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Statistical analysis issues and suggestions with the ΔΔCt method about 1 year ago

AI Summary: This GitHub issue highlights two statistical concerns in a qPCR analysis tool using the ΔΔCt method. Firstly, it points out that performing ANOVA directly on right-skewed fold change values (2^-ΔΔCt) is statistically inappropriate. Secondly, it notes that the 'Technical Replicate Handling' UI option currently has no effect on calculations, suggesting it should be implemented or simplified based on MIQE 2.0 guidelines.

Complexity: 2/5

enhancement good first issue

mianaz/qpcr_tool

Shiny App for quick analysis of qPCR data using ddCt method