Open Issues Need Help
View All on GitHub Statistical analysis issues and suggestions with the ΔΔCt method about 2 months ago
AI Summary: This GitHub issue highlights two statistical concerns in a qPCR analysis tool using the ΔΔCt method. Firstly, it points out that performing ANOVA directly on right-skewed fold change values (2^-ΔΔCt) is statistically inappropriate. Secondly, it notes that the 'Technical Replicate Handling' UI option currently has no effect on calculations, suggesting it should be implemented or simplified based on MIQE 2.0 guidelines.
Complexity:
2/5
enhancement good first issue